› 3l2c

X-ray diffraction
1.87Å resolution

Crystal Structure of the DNA Binding Domain of FOXO4 Bound to DNA

Released:
DOI: 10.2210/pdb3l2c/pdb
PDB entry 3l2c coloured by chain, front view.
PDB entry 3l2c coloured by chain, side view.
PDB entry 3l2c coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the human FOXO4-DBD-DNA complex at 1.9 Å resolution reveals new details of FOXO binding to the DNA.
Boura E, Rezabkova L, Brynda J, Obsilova V, Obsil T
Acta Crystallogr D Biol Crystallogr 66 1351-7 (2010)
PMID: 21123876

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116514 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Forkhead box protein O4 Chain: A
Molecule details ›
Chain: A
Length: 110 amino acids
Theoretical weight: 12.28 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P98177 (Residues: 86-187; Coverage: 20%)
Gene names: AFX, AFX1, FOXO4, MLLT7
Sequence domains: Forkhead domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
FOXO consensus binding sequence, plus strand Chain: B
Molecule details ›
Chain: B
Length: 13 nucleotides
Theoretical weight: 3.94 KDa
Source organism: Homo sapiens
Expression system: Not provided
FOXO consensus binding sequence, minus strand Chain: C
Molecule details ›
Chain: C
Length: 13 nucleotides
Theoretical weight: 4 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR591
Spacegroup: C2221
Unit cell:
a: 40.81Å b: 71.7Å c: 131.86Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.193 0.228
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

6 review citations

Tissue-Specific Metabolic Regulation of FOXO-Binding Protein: FOXO Does Not Act Alone.
Kodani et al. (2020)
5 more

11 mentions without citation

Regulation of cellular senescence via the FOXO4-p53 axis.
Bourgeois et al. (2018)
10 more