› 3l4c

X-ray diffraction
2.37Å resolution

Structural basis of membrane-targeting by Dock180

Released:
DOI: 10.2210/pdb3l4c/pdb
PDB entry 3l4c coloured by chain, front view.
PDB entry 3l4c coloured by chain, side view.
PDB entry 3l4c coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of membrane targeting by the Dock180 family of Rho family guanine exchange factors (Rho-GEFs).
Premkumar L, Bobkov AA, Patel M, Jaroszewski L, Bankston LA, Stec B, Vuori K, Côté JF, Liddington RC
J Biol Chem 285 13211-22 (2010)
PMID: 20167601

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171955 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dedicator of cytokinesis protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 220 amino acids
Theoretical weight: 24.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14185 (Residues: 422-619; Coverage: 11%)
Gene name: DOCK1
Sequence domains: C2 domain in Dock180 and Zizimin proteins
Structure domains: C2 domain

Ligands and Environments

1 bound ligand:
Ligand BME 2 x BME
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P21
Unit cell:
a: 46.995Å b: 63.529Å c: 63.421Å
α: 90° β: 109.28° γ: 90°
R-values:
R R work R free
0.216 0.216 0.269
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Crossroads of PI3K and Rac pathways.
Campa et al. (2015)
14 more

8 mentions without citation

Conditional peripheral membrane proteins: facing up to limited specificity.
Moravcevic et al. (2012)
7 more