› 3l4y

X-ray diffraction
1.8Å resolution

Crystal complex of N-terminal Human Maltase-Glucoamylase with NR4-8II

Released:
DOI: 10.2210/pdb3l4y/pdb
PDB entry 3l4y coloured by chain, front view.
PDB entry 3l4y coloured by chain, side view.
PDB entry 3l4y coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
New glucosidase inhibitors from an ayurvedic herbal treatment for type 2 diabetes: structures and inhibition of human intestinal maltase-glucoamylase with compounds from Salacia reticulata.
Sim L, Jayakanthan K, Mohan S, Nasi R, Johnston BD, Pinto BM, Rose DR
Biochemistry 49 443-51 (2010)
PMID: 20039683

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128929 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Maltase-glucoamylase Chain: A
Molecule details ›
Chain: A
Length: 875 amino acids
Theoretical weight: 99.28 KDa
Source organism: Homo sapiens
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: O43451 (Residues: 87-954; Coverage: 32%)
Gene names: MGA, MGAM, MGAML
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
4 bound ligands:
Ligand NR4 1 x NR4
Ligand NAG 2 x NAG
Ligand GOL 5 x GOL
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P212121
Unit cell:
a: 87.128Å b: 109.358Å c: 109.519Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.174 0.214
Expression system: Drosophila melanogaster

Quick links

Citations

9 review citations

Synthesis and biological activity of naturally occurring α-glucosidase inhibitors.
Wardrop et al. (2010)
8 more

11 mentions without citation

Evaluation of the Anti-Diabetic Activity of Some Common Herbs and Spices: Providing New Insights with Inverse Virtual Screening.
Pereira et al. (2019)
10 more