› 3l9k

X-ray diffraction
3Å resolution

Insights into dynein assembly from a dynein intermediate chain-light chain roadblock structure

Released:
DOI: 10.2210/pdb3l9k/pdb
PDB entry 3l9k coloured by chain, front view.
PDB entry 3l9k coloured by chain, side view.
PDB entry 3l9k coloured by chain, top view.
Source organism: Drosophila melanogaster
Entry authors: Hall J, Karplus PA, Barbar E

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-173183 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Dynein light chain roadblock Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 97 amino acids
Theoretical weight: 10.84 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7KMS3 (Residues: 1-97; Coverage: 100%)
Gene names: CG10751, DLC, Dmel\CG10751, Dmel_CG10751, LC7, l(2)k10408, robl, roblk
Sequence domains: Roadblock/LC7 domain
Structure domains: Dynein light chain 2a, cytoplasmic
Cytoplasmic dynein 1 intermediate chain Chains: W, X, Y, Z
Molecule details ›
Chains: W, X, Y, Z
Length: 38 amino acids
Theoretical weight: 4.49 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q24246 (Residues: 242-279; Coverage: 6%)
Gene names: CG18000, Cdic, Dic19B, sw

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P32
Unit cell:
a: 107.18Å b: 107.18Å c: 65.41Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.228 0.227 0.249
Expression system: Escherichia coli BL21(DE3)

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