› 3l9q

X-ray diffraction
1.7Å resolution

Crystal structure of human polymerase alpha-primase p58 iron-sulfur cluster domain

Released:
DOI: 10.2210/pdb3l9q/pdb
PDB entry 3l9q coloured by chain, front view.
PDB entry 3l9q coloured by chain, side view.
PDB entry 3l9q coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Insights into eukaryotic DNA priming from the structure and functional interactions of the 4Fe-4S cluster domain of human DNA primase.
Vaithiyalingam S, Warren EM, Eichman BF, Chazin WJ
Proc Natl Acad Sci U S A 107 13684-9 (2010)
PMID: 20643958

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155976 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA primase large subunit Chains: A, B
Molecule details ›
Chains: A, B
Length: 195 amino acids
Theoretical weight: 22.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P49643 (Residues: 272-464; Coverage: 38%)
Gene names: PRIM2, PRIM2A
Sequence domains: Eukaryotic and archaeal DNA primase, large subunit

Ligands and Environments

2 bound ligands:
Ligand SF4 2 x SF4
Ligand SO4 5 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: C2
Unit cell:
a: 109.441Å b: 53.05Å c: 88.844Å
α: 90° β: 115.09° γ: 90°
R-values:
R R work R free
0.132 0.13 0.169
Expression system: Escherichia coli

Quick links

Citations

9 review citations

DNA helicase and helicase-nuclease enzymes with a conserved iron-sulfur cluster.
Wu et al. (2012)
8 more

11 mentions without citation

Emerging critical roles of Fe-S clusters in DNA replication and repair.
Fuss et al. (2015)
10 more