› 3lb6

X-ray diffraction
3.05Å resolution

The structure of IL-13 in complex with IL-13Ralpha2

Released:
DOI: 10.2210/pdb3lb6/pdb
PDB entry 3lb6 coloured by chain, front view.
PDB entry 3lb6 coloured by chain, side view.
PDB entry 3lb6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular basis for shared cytokine recognition revealed in the structure of an unusually high affinity complex between IL-13 and IL-13Ralpha2.
Lupardus PJ, Birnbaum ME, Garcia KC
Structure 18 332-42 (2010)
PMID: 20223216

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152844 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Interleukin-13 Chain: A
Molecule details ›
Chain: A
Length: 132 amino acids
Theoretical weight: 14.33 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P35225 (Residues: 15-146; Coverage: 100%)
Gene names: IL13, NC30
Sequence domains: Interleukin-13
Structure domains: Growth Hormone; Chain: A;
Interleukin-13 receptor subunit alpha-2 Chain: C
Molecule details ›
Chain: C
Length: 380 amino acids
Theoretical weight: 44.25 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q14627 (Residues: 1-380; Coverage: 100%)
Gene names: IL13R, IL13RA2
Sequence domains: Interleukin-6 receptor alpha chain, binding
Structure domains: Immunoglobulins
Interleukin-13 Chain: B
Molecule details ›
Chain: B
Length: 132 amino acids
Theoretical weight: 14.36 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P35225 (Residues: 15-146; Coverage: 100%)
Gene names: IL13, NC30
Sequence domains: Interleukin-13
Structure domains: Growth Hormone; Chain: A;
Interleukin-13 receptor subunit alpha-2 Chain: D
Molecule details ›
Chain: D
Length: 380 amino acids
Theoretical weight: 44.25 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q14627 (Residues: 1-380; Coverage: 100%)
Gene names: IL13R, IL13RA2
Sequence domains: Interleukin-6 receptor alpha chain, binding
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand NAG 2 x NAG
Ligand CA 2 x CA
1 modified residue:
Ligand MLY 5 x MLY

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Unit cell:
a: 73.37Å b: 86.57Å c: 166.788Å
α: 90° β: 96.77° γ: 90°
R-values:
R R work R free
0.222 0.219 0.269
Expression system: Trichoplusia ni

Quick links

Citations

22 review citations

Alternative activation of macrophages: mechanism and functions.
Gordon et al. (2010)
21 more

11 mentions without citation

The IL13α 2R paves the way for anti-glioma nanotherapy.
Ulasov et al. (2023)
10 more