› 3lbn

X-ray diffraction
1.86Å resolution

Ras soaked in Magnesium Acetate

Released:
DOI: 10.2210/pdb3lbn/pdb
PDB entry 3lbn coloured by chain, front view.
PDB entry 3lbn coloured by chain, side view.
PDB entry 3lbn coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Allosteric modulation of Ras positions Q61 for a direct role in catalysis.
Buhrman G, Holzapfel G, Fetics S, Mattos C
Proc Natl Acad Sci U S A 107 4931-6 (2010)
PMID: 20194776

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo trimer
Assembly name:
PDBe Complex ID:
PDB-CPX-133956 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTPase HRas, N-terminally processed Chain: A
Molecule details ›
Chain: A
Length: 166 amino acids
Theoretical weight: 18.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01112 (Residues: 1-166; Coverage: 88%)
Gene names: HRAS, HRAS1
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand GNP 1 x GNP
Ligand CA 1 x CA
Ligand MG 3 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: R32
Unit cell:
a: 88.502Å b: 88.502Å c: 135.005Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.187 0.182 0.224
Expression system: Escherichia coli

Quick links

Citations

42 review citations

RAS oncogenes: weaving a tumorigenic web.
Pylayeva-Gupta et al. (2011)
41 more

4 mentions without citation

Allosteric modulation of Ras positions Q61 for a direct role in catalysis.
Buhrman et al. (2010)
3 more