› 3lck

X-ray diffraction
1.7Å resolution

THE KINASE DOMAIN OF HUMAN LYMPHOCYTE KINASE (LCK), ACTIVATED FORM (AUTO-PHOSPHORYLATED ON TYR394)

Released:
DOI: 10.2210/pdb3lck/pdb
PDB entry 3lck coloured by chain, front view.
PDB entry 3lck coloured by chain, side view.
PDB entry 3lck coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for activation of human lymphocyte kinase Lck upon tyrosine phosphorylation.
Yamaguchi H, Hendrickson WA
Nature 384 484-9 (1996)
PMID: 8945479

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138948 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein kinase Lck Chain: A
Molecule details ›
Chain: A
Length: 271 amino acids
Theoretical weight: 31.43 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P06239 (Residues: 231-501; Coverage: 53%)
Gene name: LCK
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
1 modified residue:
Ligand PTR 1 x PTR

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P212121
Unit cell:
a: 42.04Å b: 73.62Å c: 91.17Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.178 0.199
Expression system: Spodoptera frugiperda

Quick links

Citations

59 review citations

Free radicals in the physiological control of cell function.
Dröge W. (2002)
58 more

57 mentions without citation

Catalytic control in the EGF receptor and its connection to general kinase regulatory mechanisms.
Jura et al. (2011)
56 more