› 3le2

X-ray diffraction
2.2Å resolution

Structure of Arabidopsis AtSerpin1. Native Stressed Conformation

Released:
DOI: 10.2210/pdb3le2/pdb
PDB entry 3le2 coloured by chain, front view.
PDB entry 3le2 coloured by chain, side view.
PDB entry 3le2 coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Arabidopsis AtSerpin1, crystal structure and in vivo interaction with its target protease RESPONSIVE TO DESICCATION-21 (RD21).
Lampl N, Budai-Hadrian O, Davydov O, Joss TV, Harrop SJ, Curmi PM, Roberts TH, Fluhr R
J Biol Chem 285 13550-60 (2010)
PMID: 20181955

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-193426 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serpin-ZX Chain: A
Molecule details ›
Chain: A
Length: 393 amino acids
Theoretical weight: 42.81 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9S7T8 (Residues: 1-391; Coverage: 100%)
Gene names: At1g47710, F16N3.3, T2E6.22
Sequence domains: Serpin (serine protease inhibitor)
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand SO4 1 x SO4
Ligand ACT 2 x ACT
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P4322
Unit cell:
a: 54.196Å b: 54.196Å c: 299.342Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.172 0.236
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Serpins flex their muscle: I. Putting the clamps on proteolysis in diverse biological systems.
Silverman et al. (2010)
11 more

8 mentions without citation

Peptide-based protease inhibitors from plants.
Hellinger et al. (2019)
7 more