› 3lfv

X-ray diffraction
2.8Å resolution

crystal structure of unliganded PDE5A GAF domain

Released:
DOI: 10.2210/pdb3lfv/pdb
PDB entry 3lfv coloured by chain, front view.
PDB entry 3lfv coloured by chain, side view.
PDB entry 3lfv coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Conformation changes, N-terminal involvement, and cGMP signal relay in the phosphodiesterase-5 GAF domain.
Wang H, Robinson H, Ke H
J Biol Chem 285 38149-56 (2010)
PMID: 20861010

Function and Biology Details

Reaction catalysed: 
Guanosine 3',5'-cyclic phosphate + H(2)O = guanosine 5'-phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131209 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cGMP-specific 3',5'-cyclic phosphodiesterase Chains: A, B
Molecule details ›
Chains: A, B
Length: 431 amino acids
Theoretical weight: 48.86 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O76074 (Residues: 98-518; Coverage: 48%)
Gene names: PDE5, PDE5A
Sequence domains: GAF domain
Structure domains: Beta-Lactamase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P3121
Unit cell:
a: 144.189Å b: 144.189Å c: 134.867Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.243 0.24 0.285
Expression system: Escherichia coli BL21

Quick links

Citations

6 review citations

Advances in targeting cyclic nucleotide phosphodiesterases.
Maurice et al. (2014)
5 more

5 mentions without citation

Conformation changes, N-terminal involvement, and cGMP signal relay in the phosphodiesterase-5 GAF domain.
Wang et al. (2010)
4 more