› 3lk2

X-ray diffraction
2.2Å resolution

Crystal structure of CapZ bound to the uncapping motif from CARMIL

Released:
DOI: 10.2210/pdb3lk2/pdb
PDB entry 3lk2 coloured by chain, front view.
PDB entry 3lk2 coloured by chain, side view.
PDB entry 3lk2 coloured by chain, top view.
Source organisms:
Primary publication:
Structural characterization of a capping protein interaction motif defines a family of actin filament regulators.
Hernandez-Valladares M, Kim T, Kannan B, Tung A, Aguda AH, Larsson M, Cooper JA, Robinson RC
Nat Struct Mol Biol 17 497-503 (2010)
PMID: 20357771

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-146479 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
F-actin-capping protein subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 286 amino acids
Theoretical weight: 33 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: P13127 (Residues: 1-286; Coverage: 100%)
Gene name: CAPZA1
Sequence domains: F-actin capping protein alpha subunit
Structure domains:
F-actin-capping protein subunit beta isoforms 1 and 2 Chain: B
Molecule details ›
Chain: B
Length: 243 amino acids
Theoretical weight: 27.32 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: P14315 (Residues: 1-243; Coverage: 88%)
Gene name: CAPZB
Sequence domains: F-actin capping protein, beta subunit
Structure domains:
F-actin-uncapping protein LRRC16A Chain: T
Molecule details ›
Chain: T
Length: 52 amino acids
Theoretical weight: 5.92 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q5VZK9 (Residues: 961-1012; Coverage: 4%)
Gene names: CARMIL, CARMIL1, LRRC16, LRRC16A

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P212121
Unit cell:
a: 54.9Å b: 71.8Å c: 154.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.209 0.268
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

14 review citations

Capping protein regulators fine-tune actin assembly dynamics.
Edwards et al. (2014)
13 more

2 mentions without citation

Structural characterization of a capping protein interaction motif defines a family of actin filament regulators.
Hernandez-Valladares et al. (2010)
1 more