› 3lne

X-ray diffraction
2Å resolution

Crystal structure of E-cadherin EC12 K14E

Released:
DOI: 10.2210/pdb3lne/pdb
PDB entry 3lne coloured by chain, front view.
PDB entry 3lne coloured by chain, side view.
PDB entry 3lne coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Two-step adhesive binding by classical cadherins.
Harrison OJ, Bahna F, Katsamba PS, Jin X, Brasch J, Vendome J, Ahlsen G, Carroll KJ, Price SR, Honig B, Shapiro L
Nat Struct Mol Biol 17 348-57 (2010)
PMID: 20190754

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140757 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cadherin-1 Chain: A
Molecule details ›
Chain: A
Length: 213 amino acids
Theoretical weight: 23.26 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P09803 (Residues: 157-369; Coverage: 25%)
Gene name: Cdh1
Sequence domains: Cadherin domain
Structure domains: Cadherins

Ligands and Environments

2 bound ligands:
Ligand CA 3 x CA
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: C2
Unit cell:
a: 130.001Å b: 48.536Å c: 54.486Å
α: 90° β: 111.63° γ: 90°
R-values:
R R work R free
0.174 0.172 0.219
Expression system: Escherichia coli

Quick links

Citations

31 review citations

E-cadherin junctions as active mechanical integrators in tissue dynamics.
Lecuit et al. (2015)
30 more

3 mentions without citation

Molecular determinants of cadherin ideal bond formation: Conformation-dependent unbinding on a multidimensional landscape.
Manibog et al. (2016)
2 more