› 3lng

X-ray diffraction
2.7Å resolution

Crystal structure of E-cadherin EC12 AA extension

Released:
DOI: 10.2210/pdb3lng/pdb
PDB entry 3lng coloured by chain, front view.
PDB entry 3lng coloured by chain, side view.
PDB entry 3lng coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Two-step adhesive binding by classical cadherins.
Harrison OJ, Bahna F, Katsamba PS, Jin X, Brasch J, Vendome J, Ahlsen G, Carroll KJ, Price SR, Honig B, Shapiro L
Nat Struct Mol Biol 17 348-57 (2010)
PMID: 20190754

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140757 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cadherin-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 215 amino acids
Theoretical weight: 23.4 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P09803 (Residues: 157-369; Coverage: 25%)
Gene name: Cdh1
Sequence domains: Cadherin domain
Structure domains: Cadherins

Ligands and Environments

1 bound ligand:
Ligand CA 6 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: C2
Unit cell:
a: 121.536Å b: 80.108Å c: 72.691Å
α: 90° β: 118.76° γ: 90°
R-values:
R R work R free
0.22 0.218 0.263
Expression system: Escherichia coli

Quick links

Citations

31 review citations

E-cadherin junctions as active mechanical integrators in tissue dynamics.
Lecuit et al. (2015)
30 more

2 mentions without citation

Structural and energetic determinants of adhesive binding specificity in type I cadherins.
Vendome et al. (2014)
1 more