› 3lo5

X-ray diffraction
2.57Å resolution

Crystal Structure of the dominant negative S17N mutant of Ras

Released:
DOI: 10.2210/pdb3lo5/pdb
PDB entry 3lo5 coloured by chain, front view.
PDB entry 3lo5 coloured by chain, side view.
PDB entry 3lo5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the dominant negative S17N mutant of Ras.
Nassar N, Singh K, Garcia-Diaz M
Biochemistry 49 1970-4 (2010)
PMID: 20131908

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133956 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTPase HRas, N-terminally processed Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 166 amino acids
Theoretical weight: 18.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01112 (Residues: 1-166; Coverage: 88%)
Gene names: HRAS, HRAS1
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand GDP 3 x GDP
Ligand CA 4 x CA
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: I222
Unit cell:
a: 92.475Å b: 102.184Å c: 117.82Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.241 0.238 0.296
Expression system: Escherichia coli

Quick links

Citations

2 review citations

The Architecture of the Rag GTPase Signaling Network.
Nicastro et al. (2017)
1 more

6 mentions without citation

K-Ras(G12C) inhibitors allosterically control GTP affinity and effector interactions.
Ostrem et al. (2013)
5 more