› 3lqa

X-ray diffraction
3.4Å resolution

Crystal structure of clade C gp120 in complex with sCD4 and 21c Fab

Released:
DOI: 10.2210/pdb3lqa/pdb
PDB entry 3lqa coloured by chain, front view.
PDB entry 3lqa coloured by chain, side view.
PDB entry 3lqa coloured by chain, top view.
Source organisms:
Primary publication:
Structure of a clade C HIV-1 gp120 bound to CD4 and CD4-induced antibody reveals anti-CD4 polyreactivity.
Diskin R, Marcovecchio PM, Bjorkman PJ
Nat Struct Mol Biol 17 608-13 (2010)
PMID: 20357769

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-208502 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
T-cell surface glycoprotein CD4 Chain: C
Molecule details ›
Chain: C
Length: 192 amino acids
Theoretical weight: 21.47 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P01730 (Residues: 26-207; Coverage: 42%)
Gene name: CD4
Sequence domains:
Structure domains: Immunoglobulins
Envelope glycoprotein gp160 Chain: G
Molecule details ›
Chain: G
Length: 332 amino acids
Theoretical weight: 36.79 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q1PHM6 (Residues: 82-293, 325-494; Coverage: 37%)
Gene name: env
Sequence domains: Envelope glycoprotein GP120
Structure domains: Human immunodeficiency virus 1, Gp160, envelope glycoprotein
Heavy chain of anti HIV Fab from human 21c antibody Chain: H
Molecule details ›
Chain: H
Length: 231 amino acids
Theoretical weight: 24.62 KDa
Source organism: Homo sapiens
Expression system: Not provided
Structure domains: Immunoglobulins
Light chain of anti HIV Fab from human 21c antibody Chain: L
Molecule details ›
Chain: L
Length: 217 amino acids
Theoretical weight: 22.82 KDa
Source organism: Homo sapiens
Expression system: Not provided
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand NAG 4 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: C2221
Unit cell:
a: 93.73Å b: 187.93Å c: 151.67Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.234 0.322
Expression systems:
  • Spodoptera frugiperda
  • Not provided

Quick links

Citations

8 review citations

Antibodies in HIV-1 vaccine development and therapy.
Klein et al. (2013)
7 more

13 mentions without citation

HIV-1 envelope glycoprotein structure.
Merk et al. (2013)
12 more