› 3lrn

X-ray diffraction
2.6Å resolution

Crystal structure of human RIG-I CTD bound to a 14 bp GC 5' ppp dsRNA

Released:
DOI: 10.2210/pdb3lrn/pdb
PDB entry 3lrn coloured by chain, front view.
PDB entry 3lrn coloured by chain, side view.
PDB entry 3lrn coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The structural basis of 5' triphosphate double-stranded RNA recognition by RIG-I C-terminal domain.
Lu C, Xu H, Ranjith-Kumar CT, Brooks MT, Hou TY, Hu F, Herr AB, Strong RK, Kao CC, Li P
Structure 18 1032-43 (2010)
PMID: 20637642

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132038 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Antiviral innate immune response receptor RIG-I Chains: A, B
Molecule details ›
Chains: A, B
Length: 121 amino acids
Theoretical weight: 14.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O95786 (Residues: 803-923; Coverage: 13%)
Gene names: DDX58, RIGI
Sequence domains: C-terminal domain of RIG-I
Structure domains: RIG-I-like receptor, C-terminal regulatory domain
RNA (5'-R(*(GTP)P*GP*CP*GP*CP*GP*CP*GP*CP*GP*CP*GP*CP*C)-3') Chains: C, D
Molecule details ›
Chains: C, D
Length: 14 nucleotides
Theoretical weight: 4.67 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
1 modified residue:
Ligand GTP 2 x GTP

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 41.49Å b: 96.33Å c: 103.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.221 0.282
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

46 review citations

Immune signaling by RIG-I-like receptors.
Loo et al. (2011)
45 more

14 mentions without citation

A structure-based model of RIG-I activation.
Kolakofsky et al. (2012)
13 more