› 3ltf

X-ray diffraction
3.2Å resolution

Crystal Structure of the Drosophila Epidermal Growth Factor Receptor ectodomain in complex with Spitz

Released:
DOI: 10.2210/pdb3ltf/pdb
PDB entry 3ltf coloured by chain, front view.
PDB entry 3ltf coloured by chain, side view.
PDB entry 3ltf coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Structural basis for negative cooperativity in growth factor binding to an EGF receptor.
Alvarado D, Klein DE, Lemmon MA
Cell 142 568-79 (2010)
PMID: 20723758

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137630 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (5 distinct):
Epidermal growth factor receptor Chains: A, C
Molecule details ›
Chains: A, C
Length: 601 amino acids
Theoretical weight: 67.97 KDa
Source organism: Drosophila melanogaster
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P04412 (Residues: 100-688; Coverage: 42%)
Gene names: CG10079, DER, Egfr, c-erbB, top
Sequence domains:
Structure domains:
Protein spitz Chains: B, D
Molecule details ›
Chains: B, D
Length: 58 amino acids
Theoretical weight: 6.73 KDa
Source organism: Drosophila melanogaster
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: Q01083 (Residues: 76-133; Coverage: 28%)
Gene names: CG10334, spi
Structure domains: Laminin

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, MAN
Carbohydrate polymer
2 bound ligands:
Ligand NAG 3 x NAG
Ligand MLI 1 x MLI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P212121
Unit cell:
a: 118.208Å b: 124.239Å c: 186.508Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.237 0.275
Expression systems:
  • Spodoptera frugiperda
  • Drosophila melanogaster

Quick links

Citations

25 review citations

The ErbB/HER family of protein-tyrosine kinases and cancer.
Roskoski R. (2014)
24 more

8 mentions without citation

The EGFR family: not so prototypical receptor tyrosine kinases.
Lemmon et al. (2014)
7 more