› 3ltg

X-ray diffraction
3.4Å resolution

Crystal structure of the Drosophila Epidermal Growth Factor Receptor ectodomain complexed with a low affinity Spitz mutant

Released:
DOI: 10.2210/pdb3ltg/pdb
PDB entry 3ltg coloured by chain, front view.
PDB entry 3ltg coloured by chain, side view.
PDB entry 3ltg coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Structural basis for negative cooperativity in growth factor binding to an EGF receptor.
Alvarado D, Klein DE, Lemmon MA
Cell 142 568-79 (2010)
PMID: 20723758

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137629 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Epidermal growth factor receptor Chains: A, C
Molecule details ›
Chains: A, C
Length: 601 amino acids
Theoretical weight: 67.97 KDa
Source organism: Drosophila melanogaster
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P04412 (Residues: 100-688; Coverage: 42%)
Gene names: CG10079, DER, Egfr, c-erbB, top
Sequence domains:
Structure domains:
Protein spitz Chain: D
Molecule details ›
Chain: D
Length: 52 amino acids
Theoretical weight: 6.01 KDa
Source organism: Drosophila melanogaster
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: Q01083 (Residues: 76-126; Coverage: 25%)
Gene names: CG10334, spi
Structure domains: Laminin

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P212121
Unit cell:
a: 73.75Å b: 120.15Å c: 274.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.408 0.406 0.427
Expression systems:
  • Spodoptera frugiperda
  • Drosophila melanogaster

Quick links

Citations

24 review citations

The ErbB/HER family of protein-tyrosine kinases and cancer.
Roskoski R. (2014)
23 more

9 mentions without citation

The EGFR family: not so prototypical receptor tyrosine kinases.
Lemmon et al. (2014)
8 more