› 3luh

X-ray diffraction
2Å resolution

Crystal structure of MID domain from hAGO2 in complex with GMP

Released:
DOI: 10.2210/pdb3luh/pdb
PDB entry 3luh coloured by chain, front view.
PDB entry 3luh coloured by chain, side view.
PDB entry 3luh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for 5'-nucleotide base-specific recognition of guide RNA by human AGO2.
Frank F, Sonenberg N, Nagar B
Nature 465 818-22 (2010)
PMID: 20505670

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphomonoester
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193864 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein argonaute-2 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 138 amino acids
Theoretical weight: 15.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UKV8 (Residues: 439-575; Coverage: 16%)
Gene names: AGO2, EIF2C2
Sequence domains: Mid domain of argonaute
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand 5GP 3 x 5GP
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P1
Unit cell:
a: 40.433Å b: 46.971Å c: 66.352Å
α: 86.06° β: 72.77° γ: 83.7°
R-values:
R R work R free
0.188 0.185 0.233
Expression system: Escherichia coli

Quick links

Citations

117 review citations

The widespread regulation of microRNA biogenesis, function and decay.
Krol et al. (2010)
116 more

1 mention without citation

Novel Insights into Guide RNA 5'-Nucleoside/Tide Binding by Human Argonaute 2.
Kalia et al. (2015)