› 3luk

X-ray diffraction
1.7Å resolution

Crystal structure of MID domain from hAGO2

Released:
DOI: 10.2210/pdb3luk/pdb
PDB entry 3luk coloured by chain, front view.
PDB entry 3luk coloured by chain, side view.
PDB entry 3luk coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for 5'-nucleotide base-specific recognition of guide RNA by human AGO2.
Frank F, Sonenberg N, Nagar B
Nature 465 818-22 (2010)
PMID: 20505670

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphomonoester
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193864 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein argonaute-2 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 138 amino acids
Theoretical weight: 15.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UKV8 (Residues: 439-575; Coverage: 16%)
Gene names: AGO2, EIF2C2
Sequence domains: Mid domain of argonaute
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand PO4 8 x PO4
Ligand GOL 1 x GOL
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P1
Unit cell:
a: 40.8Å b: 47.851Å c: 66.846Å
α: 85.9° β: 72.84° γ: 82.86°
R-values:
R R work R free
0.191 0.188 0.232
Expression system: Escherichia coli

Quick links

Citations

117 review citations

The widespread regulation of microRNA biogenesis, function and decay.
Krol et al. (2010)
116 more

1 mention without citation

Novel Insights into Guide RNA 5'-Nucleoside/Tide Binding by Human Argonaute 2.
Kalia et al. (2015)