› 3lv3

X-ray diffraction
1.94Å resolution

Crystal structure of HLA-B*2705 complexed with a peptide derived from the human voltage-dependent calcium channel alpha1 subunit (residues 513-521)

Released:
DOI: 10.2210/pdb3lv3/pdb
PDB entry 3lv3 coloured by chain, front view.
PDB entry 3lv3 coloured by chain, side view.
PDB entry 3lv3 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Loss of recognition by cross-reactive T cells and its relation to a C-terminus-induced conformational reorientation of an HLA-B*2705-bound peptide.
Loll B, Rückert C, Hee CS, Saenger W, Uchanska-Ziegler B, Ziegler A
Protein Sci 20 278-90 (2011)
PMID: 21280120

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-135241 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
HLA class I histocompatibility antigen, B alpha chain Chain: A
Molecule details ›
Chain: A
Length: 276 amino acids
Theoretical weight: 31.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P01889 (Residues: 25-300; Coverage: 82%)
Gene names: HLA-B, HLAB
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Voltage-dependent L-type calcium channel subunit alpha-1D Chain: C
Molecule details ›
Chain: C
Length: 9 amino acids
Theoretical weight: 1.38 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q01668 (Residues: 502-510; Coverage: 0%)
Gene names: CACH3, CACN4, CACNA1D, CACNL1A2, CCHL1A2

Ligands and Environments

1 bound ligand:
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21
Unit cell:
a: 56.29Å b: 54.614Å c: 69.834Å
α: 90° β: 99.33° γ: 90°
R-values:
R R work R free
0.178 0.176 0.224
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

1 review citation

Three-Dimensional Portable Document Format (3D PDF) in Clinical Communication and Biomedical Sciences: Systematic Review of Applications, Tools, and Protocols.
Newe et al. (2018)
2 other articles

3 mentions without citation

Major histocompatibility complex associations of ankylosing spondylitis are complex and involve further epistasis with ERAP1.
Cortes et al. (2015)
2 more