› 3lve

X-ray diffraction
2Å resolution

LEN Q38E MUTANT: A DOMAIN FLIP FROM A SINGLE AMINO ACID SUBSTITUTION

Released:
DOI: 10.2210/pdb3lve/pdb
PDB entry 3lve coloured by chain, front view.
PDB entry 3lve coloured by chain, side view.
PDB entry 3lve coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A domain flip as a result of a single amino-acid substitution.
Pokkuluri PR, Huang DB, Raffen R, Cai X, Johnson G, Stevens PW, Stevens FJ, Schiffer M
Structure 6 1067-73 (1998)
PMID: 9739086

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138999 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Immunoglobulin kappa variable 4-1 Chain: A
Molecule details ›
Chain: A
Length: 114 amino acids
Theoretical weight: 12.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P06312 (Residues: 21-121; Coverage: 100%)
Gene name: IGKV4-1
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P4222
Unit cell:
a: 65.3Å b: 65.3Å c: 51.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.176 0.248
Expression system: Escherichia coli

Quick links

Citations

3 review citations

An improved model of association for VH-VL immunoglobulin domains: asymmetries between VH and VL in the packing of some interface residues.
Vargas-Madrazo et al. (2003)
2 more