› 3lvr

X-ray diffraction
3.38Å resolution

The crystal structure of ASAP3 in complex with Arf6 in transition state soaked with Calcium

Released:
DOI: 10.2210/pdb3lvr/pdb
PDB entry 3lvr coloured by chain, front view.
PDB entry 3lvr coloured by chain, side view.
PDB entry 3lvr coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The structure of an Arf-ArfGAP complex reveals a Ca2+ regulatory mechanism.
Ismail SA, Vetter IR, Sot B, Wittinghofer A
Cell 141 812-21 (2010)
PMID: 20510928

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158603 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ADP-ribosylation factor 6; Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 3 Chain: E
Molecule details ›
Chain: E
Length: 497 amino acids
Theoretical weight: 54.83 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8TDY4 (Residues: 416-698, 699-702; Coverage: 32%)
  • Canonical: P62330 (Residues: 9-175; Coverage: 95%)
Gene names: ARF6, ASAP3, DDEFL1, UPLC1
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand ZN 1 x ZN
Ligand MG 1 x MG
Ligand GDP 1 x GDP
Ligand AF3 1 x AF3
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P62
Unit cell:
a: 146.45Å b: 146.45Å c: 49.62Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.211 0.207 0.281
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Regulation of small GTPases by GEFs, GAPs, and GDIs.
Cherfils et al. (2013)
11 more

5 mentions without citation

Invited review: Small GTPases and their GAPs.
Mishra et al. (2016)
4 more