› 3lwt

X-ray diffraction
1.96Å resolution

Crystal structure of the Yeast Sac1: Implications for its phosphoinositide phosphatase function

Released:
DOI: 10.2210/pdb3lwt/pdb
PDB entry 3lwt coloured by chain, front view.
PDB entry 3lwt coloured by chain, side view.
PDB entry 3lwt coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Crystal structure of the yeast Sac1: implications for its phosphoinositide phosphatase function.
Manford A, Xia T, Saxena AK, Stefan C, Hu F, Emr SD, Mao Y
EMBO J 29 1489-98 (2010)
PMID: 20389282

Function and Biology Details

Reaction catalysed: 
1-phosphatidyl-1D-myo-inositol 3-phosphate + H(2)O = 1-phosphatidyl-1D-myo-inositol + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-152281 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphatidylinositol-3-phosphatase SAC1 Chain: X
Molecule details ›
Chain: X
Length: 505 amino acids
Theoretical weight: 57.74 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P32368 (Residues: 1-504; Coverage: 81%)
Gene names: RSD1, SAC1, YKL212W
Sequence domains: SacI homology domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: I222
Unit cell:
a: 86.158Å b: 94.732Å c: 155.439Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.199 0.243
Expression system: Escherichia coli

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Citations

27 review citations

Phosphoinositides: tiny lipids with giant impact on cell regulation.
Balla T. (2013)
26 more

9 mentions without citation

The structure of phosphoinositide phosphatases: Insights into substrate specificity and catalysis.
Hsu et al. (2015)
8 more