› 3m61

X-ray diffraction
1.68Å resolution

Crystal structure of complex of urokinase and a upain-1 variant(W3A) in pH4.6 condition

Released:
DOI: 10.2210/pdb3m61/pdb
PDB entry 3m61 coloured by chain, front view.
PDB entry 3m61 coloured by chain, side view.
PDB entry 3m61 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Jiang L, Yuan C, Wind T, Andreasen PA, Chen L, Meehan EJ, Huang M

Function and Biology Details

Reaction catalysed: 
Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133257 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Urokinase-type plasminogen activator chain B Chain: U
Molecule details ›
Chain: U
Length: 253 amino acids
Theoretical weight: 28.44 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P00749 (Residues: 179-431; Coverage: 62%)
Gene name: PLAU
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
upain-1 W3A Chain: P
Molecule details ›
Chain: P
Length: 12 amino acids
Theoretical weight: 1.38 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: R3
Unit cell:
a: 121.705Å b: 121.705Å c: 43.128Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.198 0.195 0.243
Expression systems:
  • Komagataella pastoris
  • Not provided

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