› 3m94

X-ray diffraction
2.05Å resolution

Complex crystal structure of Ascaris suum eIF4E-3 with m2,2,7G cap

Released:
DOI: 10.2210/pdb3m94/pdb
PDB entry 3m94 coloured by chain, front view.
PDB entry 3m94 coloured by chain, side view.
PDB entry 3m94 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for nematode eIF4E binding an m(2,2,7)G-Cap and its implications for translation initiation.
Liu W, Jankowska-Anyszka M, Piecyk K, Dickson L, Wallace A, Niedzwiecka A, Stepinski J, Stolarski R, Darzynkiewicz E, Kieft J, Zhao R, Jones DN, Davis RE
Nucleic Acids Res 39 8820-32 (2011)
PMID: 21965542

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171744 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
eIF-4F 25 kDa subunit Chain: A
Molecule details ›
Chain: A
Length: 189 amino acids
Theoretical weight: 22.17 KDa
Source organism: Ascaris suum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6PKX2 (Residues: 48-236; Coverage: 80%)
Sequence domains: Eukaryotic initiation factor 4E
Structure domains: RNA Cap, Translation Initiation Factor Eif4e
Eukaryotic translation initiation factor 4E-binding protein 1 Chain: C
Molecule details ›
Chain: C
Length: 17 amino acids
Theoretical weight: 2.14 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q13541 (Residues: 51-67; Coverage: 14%)
Gene name: EIF4EBP1

Ligands and Environments

2 bound ligands:
Ligand M7M 1 x M7M
Ligand ACE 1 x ACE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: I41
Unit cell:
a: 104.977Å b: 104.977Å c: 46.906Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.204 0.244
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

5 review citations

The oncogene eIF4E: using biochemical insights to target cancer.
Carroll et al. (2013)
4 more

1 mention without citation

Structural basis for nematode eIF4E binding an m(2,2,7)G-Cap and its implications for translation initiation.
Liu et al. (2011)