› 3mdj

X-ray diffraction
2.95Å resolution

ER Aminopeptidase, ERAP1, Bound to the Zinc Aminopeptidase Inhibitor, Bestatin

Released:
DOI: 10.2210/pdb3mdj/pdb
PDB entry 3mdj coloured by chain, front view.
PDB entry 3mdj coloured by chain, side view.
PDB entry 3mdj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for antigenic peptide precursor processing by the endoplasmic reticulum aminopeptidase ERAP1.
Nguyen TT, Chang SC, Evnouchidou I, York IA, Zikos C, Rock KL, Goldberg AL, Stratikos E, Stern LJ
Nat Struct Mol Biol 18 604-13 (2011)
PMID: 21478864

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192727 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Endoplasmic reticulum aminopeptidase 1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 921 amino acids
Theoretical weight: 105.24 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9NZ08 (Residues: 37-939; Coverage: 96%)
Gene names: APPILS, ARTS1, ERAP1, KIAA0525, UNQ584/PRO1154
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
3 bound ligands:
Ligand ZN 3 x ZN
Ligand BES 3 x BES
Ligand NAG 3 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 71.029Å b: 234.635Å c: 95.86Å
α: 90° β: 103.59° γ: 90°
R-values:
R R work R free
0.202 0.199 0.264
Expression system: Spodoptera frugiperda

Quick links

Citations

37 review citations

Towards a systems understanding of MHC class I and MHC class II antigen presentation.
Neefjes et al. (2011)
36 more

20 mentions without citation

A role for naturally occurring alleles of endoplasmic reticulum aminopeptidases in tumor immunity and cancer pre-disposition.
Stratikos et al. (2014)
19 more