› 3mf0

X-ray diffraction
3.1Å resolution

Crystal structure of PDE5A GAF domain (89-518)

Released:
DOI: 10.2210/pdb3mf0/pdb
PDB entry 3mf0 coloured by chain, front view.
PDB entry 3mf0 coloured by chain, side view.
PDB entry 3mf0 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Conformation changes, N-terminal involvement, and cGMP signal relay in the phosphodiesterase-5 GAF domain.
Wang H, Robinson H, Ke H
J Biol Chem 285 38149-56 (2010)
PMID: 20861010

Function and Biology Details

Reaction catalysed: 
Guanosine 3',5'-cyclic phosphate + H(2)O = guanosine 5'-phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131209 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cGMP-specific 3',5'-cyclic phosphodiesterase Chains: A, B
Molecule details ›
Chains: A, B
Length: 432 amino acids
Theoretical weight: 48.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O76074 (Residues: 89-518; Coverage: 49%)
Gene names: PDE5, PDE5A
Sequence domains: GAF domain
Structure domains: Beta-Lactamase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A, null
Spacegroup: P3121
Unit cell:
a: 144.534Å b: 144.534Å c: 135.008Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.235 0.232 0.289
Expression system: Escherichia coli BL21

Quick links

Citations

6 review citations

Advances in targeting cyclic nucleotide phosphodiesterases.
Maurice et al. (2014)
5 more

8 mentions without citation

Biosynthesis of the Pseudomonas aeruginosa Extracellular Polysaccharides, Alginate, Pel, and Psl.
Franklin et al. (2011)
7 more