3mfk

X-ray diffraction
3Å resolution

Ets1 complex with stromelysin-1 promoter DNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-116562 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Protein C-ets-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 162 amino acids
Theoretical weight: 18.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P14921 (Residues: 280-441; Coverage: 37%)
Gene names: ETS1, EWSR2
Sequence domains: Ets-domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
stromelysin-1 promoter DNA Chain: C
Molecule details ›
Chain: C
Length: 16 nucleotides
Theoretical weight: 4.91 KDa
Source organism: Homo sapiens
Expression system: Not provided
stromelysin-1 promoter DNA Chain: D
Molecule details ›
Chain: D
Length: 16 nucleotides
Theoretical weight: 4.88 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: C2
Unit cell:
a: 92.812Å b: 99.153Å c: 76.496Å
α: 90° β: 108.14° γ: 90°
R-values:
R R work R free
0.243 0.243 0.289
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided