3mhr

X-ray diffraction
1.15Å resolution

14-3-3 sigma in complex with YAP pS127-peptide

Released:
Source organism: Homo sapiens
Primary publication:
Structure of a 14-3-3σ-YAP phosphopeptide complex at 1.15 A resolution.
Acta Crystallogr Sect F Struct Biol Cryst Commun 66 978-84 (2010)
PMID: 20823509

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-152127 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein sigma Chain: A
Molecule details ›
Chain: A
Length: 236 amino acids
Theoretical weight: 26.56 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P31947 (Residues: 1-231; Coverage: 93%)
Gene names: HME1, SFN
Sequence domains: 14-3-3 protein
Structure domains: 14-3-3 domain
Transcriptional coactivator YAP1 Chain: P
Molecule details ›
Chain: P
Length: 10 amino acids
Theoretical weight: 1.14 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P46937 (Residues: 124-133; Coverage: 2%)
Gene names: YAP1, YAP65

Ligands and Environments

2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: C2221
Unit cell:
a: 82.27Å b: 112.11Å c: 62.85Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.128 0.126 0.153
Expression systems:
  • Escherichia coli
  • Not provided