› 3mi9

X-ray diffraction
2.1Å resolution

Crystal structure of HIV-1 Tat complexed with human P-TEFb

Released:
DOI: 10.2210/pdb3mi9/pdb
PDB entry 3mi9 coloured by chain, front view.
PDB entry 3mi9 coloured by chain, side view.
PDB entry 3mi9 coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of HIV-1 Tat complexed with human P-TEFb.
Tahirov TH, Babayeva ND, Varzavand K, Cooper JJ, Sedore SC, Price DH
Nature 465 747-51 (2010)
PMID: 20535204

Function and Biology Details

Reactions catalysed: 
ATP + a protein = ADP + a phosphoprotein
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130018 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Cyclin-dependent kinase 9 Chain: A
Molecule details ›
Chain: A
Length: 351 amino acids
Theoretical weight: 40.69 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P50750 (Residues: 1-345; Coverage: 93%)
Gene names: CDC2L4, CDK9, TAK
Sequence domains: Protein kinase domain
Structure domains:
Cyclin-T1 Chain: B
Molecule details ›
Chain: B
Length: 266 amino acids
Theoretical weight: 30.88 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O60563 (Residues: 1-266; Coverage: 37%)
Gene name: CCNT1
Sequence domains:
Structure domains: Cyclin-like
Protein Tat Chain: C
Molecule details ›
Chain: C
Length: 86 amino acids
Theoretical weight: 9.85 KDa
Source organism: HIV-1 M:B_HXB2R
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P04608 (Residues: 1-86; Coverage: 100%)
Gene name: tat
Sequence domains: Transactivating regulatory protein (Tat)

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
1 modified residue:
Ligand TPO 1 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P3221
Unit cell:
a: 132.306Å b: 132.306Å c: 95.358Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.219 0.219 0.264
Expression system: Spodoptera frugiperda

Quick links

Citations

56 review citations

RNA polymerase II elongation control.
Zhou et al. (2012)
55 more

33 mentions without citation

Structural insights into the functional diversity of the CDK-cyclin family.
Wood et al. (2018)
32 more