› 3mia

X-ray diffraction
3Å resolution

Crystal structure of HIV-1 Tat complexed with ATP-bound human P-TEFb

Released:
DOI: 10.2210/pdb3mia/pdb
PDB entry 3mia coloured by chain, front view.
PDB entry 3mia coloured by chain, side view.
PDB entry 3mia coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of HIV-1 Tat complexed with human P-TEFb.
Tahirov TH, Babayeva ND, Varzavand K, Cooper JJ, Sedore SC, Price DH
Nature 465 747-51 (2010)
PMID: 20535204

Function and Biology Details

Reactions catalysed: 
ATP + a protein = ADP + a phosphoprotein
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130018 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Cyclin-dependent kinase 9 Chain: A
Molecule details ›
Chain: A
Length: 351 amino acids
Theoretical weight: 40.69 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P50750 (Residues: 1-345; Coverage: 93%)
Gene names: CDC2L4, CDK9, TAK
Sequence domains: Protein kinase domain
Structure domains:
Cyclin-T1 Chain: B
Molecule details ›
Chain: B
Length: 266 amino acids
Theoretical weight: 30.88 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O60563 (Residues: 1-266; Coverage: 37%)
Gene name: CCNT1
Sequence domains:
Structure domains: Cyclin-like
Protein Tat Chain: C
Molecule details ›
Chain: C
Length: 86 amino acids
Theoretical weight: 9.85 KDa
Source organism: HIV-1 M:B_HXB2R
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P04608 (Residues: 1-86; Coverage: 100%)
Gene name: tat
Sequence domains: Transactivating regulatory protein (Tat)

Ligands and Environments

3 bound ligands:
Ligand ANP 1 x ANP
Ligand MG 1 x MG
Ligand ZN 2 x ZN
1 modified residue:
Ligand TPO 1 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P3221
Unit cell:
a: 134.286Å b: 134.286Å c: 97.185Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.219 0.219 0.287
Expression system: Spodoptera frugiperda

Quick links

Citations

57 review citations

RNA polymerase II elongation control.
Zhou et al. (2012)
56 more

21 mentions without citation

The control of HIV transcription: keeping RNA polymerase II on track.
Ott et al. (2011)
20 more