› 3mo0

X-ray diffraction
2.78Å resolution

Human G9a-like (GLP, also known as EHMT1) in complex with inhibitor E11

Released:
DOI: 10.2210/pdb3mo0/pdb
PDB entry 3mo0 coloured by chain, front view.
PDB entry 3mo0 coloured by chain, side view.
PDB entry 3mo0 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Adding a lysine mimic in the design of potent inhibitors of histone lysine methyltransferases.
Chang Y, Ganesh T, Horton JR, Spannhoff A, Liu J, Sun A, Zhang X, Bedford MT, Shinkai Y, Snyder JP, Cheng X
J Mol Biol 400 1-7 (2010)
PMID: 20434463

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + a [histone H3]-L-lysine(9) = S-adenosyl-L-homocysteine + a [histone H3]-N(6)-methyl-L-lysine(9)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-190698 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Histone-lysine N-methyltransferase EHMT1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 285 amino acids
Theoretical weight: 32.83 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9H9B1 (Residues: 982-1266; Coverage: 22%)
Gene names: EHMT1, EUHMTASE1, GLP, KIAA1876, KMT1D
Sequence domains:
Structure domains: SET domain

Ligands and Environments

4 bound ligands:
Ligand SAH 2 x SAH
Ligand E11 3 x E11
Ligand ZN 10 x ZN
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 47.3Å b: 94.5Å c: 138.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.225 0.306
Expression system: Escherichia coli

Quick links

Citations

25 review citations

Epigenetic protein families: a new frontier for drug discovery.
Arrowsmith et al. (2012)
24 more

9 mentions without citation

Modulation of epigenetic targets for anticancer therapy: clinicopathological relevance, structural data and drug discovery perspectives.
Andreoli et al. (2013)
8 more