› 3mp1

X-ray diffraction
2.6Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142526 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Maltose/maltodextrin-binding periplasmic protein; SAGA-associated factor 29 Chain: A
Molecule details ›
Chain: A
Length: 522 amino acids
Theoretical weight: 57.52 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AEX9 (Residues: 27-392; Coverage: 99%)
  • Canonical: P25554 (Residues: 105-259; Coverage: 60%)
Gene names: JW3994, SGF29, YCL010C, YCL10C, b4034, malE
Sequence domains:
Structure domains:
Histone H3.1 Chain: P
Molecule details ›
Chain: P
Length: 5 amino acids
Theoretical weight: 647 Da
Source organism: synthetic construct
Expression system: Not provided
UniProt:
  • Canonical: P68431 (Residues: 2-6; Coverage: 4%)
Gene names: H3C1, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, H3FB, H3FC HIST1H3C, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
1 bound ligand:
Ligand ACT 1 x ACT
1 modified residue:
Ligand M3L 1 x M3L

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 50.238Å b: 84.845Å c: 119.15Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.223 0.26
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

50 review citations

Perceiving the epigenetic landscape through histone readers.
Musselman et al. (2012)
49 more

3 mentions without citation

Crystal structures of MBP fusion proteins.
Waugh DS. (2016)
2 more