› 3mq9

X-ray diffraction
2.8Å resolution

Crystal Structure of Ectodomain Mutant of BST-2/Tetherin/CD317 Fused to MBP

Released:
DOI: 10.2210/pdb3mq9/pdb
PDB entry 3mq9 coloured by chain, front view.
PDB entry 3mq9 coloured by chain, side view.
PDB entry 3mq9 coloured by chain, top view.
Source organisms:
Primary publication:
Structural insight into the mechanisms of enveloped virus tethering by tetherin.
Yang H, Wang J, Jia X, McNatt MW, Zang T, Pan B, Meng W, Wang HW, Bieniasz PD, Xiong Y
Proc Natl Acad Sci U S A 107 18428-32 (2010)
PMID: 20940320

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142558 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bone marrow stromal antigen 2; Maltose/maltodextrin-binding periplasmic protein Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 471 amino acids
Theoretical weight: 52.19 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEX9 (Residues: 27-395; Coverage: 100%)
  • Canonical: Q10589 (Residues: 66-139; Coverage: 41%)
Gene names: BST2, JW3994, b4034, malE
Sequence domains:
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 24 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 94.502Å b: 202.443Å c: 107.277Å
α: 90° β: 90.41° γ: 90°
R-values:
R R work R free
0.233 0.231 0.279
Expression system: Escherichia coli

Quick links

Citations

21 review citations

HIV Restriction Factors and Mechanisms of Evasion.
Malim et al. (2012)
20 more

3 mentions without citation

Crystal structures of MBP fusion proteins.
Waugh DS. (2016)
2 more