› 3mtr

X-ray diffraction
1.8Å resolution

Crystal structure of the Ig5-FN1 tandem of human NCAM

Released:
DOI: 10.2210/pdb3mtr/pdb
PDB entry 3mtr coloured by chain, front view.
PDB entry 3mtr coloured by chain, side view.
PDB entry 3mtr coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure and mutagenesis of neural cell adhesion molecule domains: evidence for flexibility in the placement of polysialic acid attachment sites.
Foley DA, Swartzentruber KG, Lavie A, Colley KJ
J Biol Chem 285 27360-27371 (2010)
PMID: 20573953

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146666 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neural cell adhesion molecule 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 215 amino acids
Theoretical weight: 23.42 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P13591 (Residues: 414-611; Coverage: 24%)
Gene names: NCAM, NCAM1
Sequence domains:
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: C2221
Unit cell:
a: 77.09Å b: 155.84Å c: 71.75Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.183 0.23
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Protein glycosylation in cancer.
Stowell et al. (2015)
2 more

7 mentions without citation

Sequences at the interface of the fifth immunoglobulin domain and first fibronectin type III repeat of the neural cell adhesion molecule are critical for its polysialylation.
Thompson et al. (2011)
6 more