› 3mtu

X-ray diffraction
2.1Å resolution

Structure of the Tropomyosin Overlap Complex from Chicken Smooth Muscle

Released:
DOI: 10.2210/pdb3mtu/pdb
PDB entry 3mtu coloured by chain, front view.
PDB entry 3mtu coloured by chain, side view.
PDB entry 3mtu coloured by chain, top view.
Source organisms:
Primary publication:
Structure of the tropomyosin overlap complex from chicken smooth muscle: insight into the diversity of N-terminal recognition.
Frye J, Klenchin VA, Rayment I
Biochemistry 49 4908-20 (2010)
PMID: 20465283

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero hexamer
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137545 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Microtubule-associated protein RP/EB family member 1; Tropomyosin alpha-1 chain Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 75 amino acids
Theoretical weight: 8.71 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P04268 (Residues: 1-29; Coverage: 10%)
  • Canonical: Q15691 (Residues: 216-257; Coverage: 16%)
Gene names: MAPRE1, TPM1
Sequence domains:
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Capsid assembly scaffolding protein; Tropomyosin alpha-1 chain Chains: E, F
Molecule details ›
Chains: E, F
Length: 77 amino acids
Theoretical weight: 8.96 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P04268 (Residues: 257-257, 267-283; Coverage: 6%)
  • Canonical: P13848 (Residues: 2-46, 51-59; Coverage: 55%)
Gene names: 7, TPM1
Sequence domains: Phi29 scaffolding protein
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

3 bound ligands:
Ligand CL 2 x CL
Ligand EDO 9 x EDO
Ligand EOH 7 x EOH
1 modified residue:
Ligand MSE 18 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: C222
Unit cell:
a: 89.131Å b: 285.328Å c: 43.457Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.201 0.241
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Tropomyosin: double helix from the protein world.
Nevzorov et al. (2011)
8 more

3 mentions without citation

Structure of the tropomyosin overlap complex from chicken smooth muscle: insight into the diversity of N-terminal recognition.
Frye et al. (2010)
2 more