› 3mud

X-ray diffraction
2.2Å resolution

Structure of the Tropomyosin Overlap Complex from Chicken Smooth Muscle

Released:
DOI: 10.2210/pdb3mud/pdb
PDB entry 3mud coloured by chain, front view.
PDB entry 3mud coloured by chain, side view.
PDB entry 3mud coloured by chain, top view.
Source organisms:
Primary publication:
Structure of the tropomyosin overlap complex from chicken smooth muscle: insight into the diversity of N-terminal recognition.
Frye J, Klenchin VA, Rayment I
Biochemistry 49 4908-20 (2010)
PMID: 20465283

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137547 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DNA repair protein XRCC4; Tropomyosin alpha-1 chain Chains: A, B
Molecule details ›
Chains: A, B
Length: 175 amino acids
Theoretical weight: 19.74 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: Q13426 (Residues: 2-135, 137-137; Coverage: 40%)
  • Canonical: P04268 (Residues: 248-248, 250-284; Coverage: 13%)
Gene names: TPM1, XRCC4
Sequence domains: DNA double-strand break repair and V(D)J recombination protein XRCC4
Structure domains:
Microtubule-associated protein RP/EB family member 1; Tropomyosin alpha-1 chain Chains: C, D
Molecule details ›
Chains: C, D
Length: 75 amino acids
Theoretical weight: 8.57 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P09493 (Residues: 1-29; Coverage: 10%)
  • Canonical: Q15691 (Residues: 215-257; Coverage: 16%)
Gene names: C15orf13, MAPRE1, TMSA, TPM1
Sequence domains:
Structure domains: Vasodilator-stimulated phosphoprotein

Ligands and Environments

2 bound ligands:
Ligand EDO 1 x EDO
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P41212
Unit cell:
a: 96.564Å b: 96.564Å c: 162.993Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.207 0.25
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Tropomodulins and Leiomodins: Actin Pointed End Caps and Nucleators in Muscles.
Fowler et al. (2017)
9 more

4 mentions without citation

Structure of the tropomyosin overlap complex from chicken smooth muscle: insight into the diversity of N-terminal recognition.
Frye et al. (2010)
3 more