› 3mva

X-ray diffraction
2.2Å resolution

Crystal structure of human MTERF1 bound to the termination sequence

Released:
DOI: 10.2210/pdb3mva/pdb
PDB entry 3mva coloured by chain, front view.
PDB entry 3mva coloured by chain, side view.
PDB entry 3mva coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Helix unwinding and base flipping enable human MTERF1 to terminate mitochondrial transcription.
Yakubovskaya E, Mejia E, Byrnes J, Hambardjieva E, Garcia-Diaz M
Cell 141 982-93 (2010)
PMID: 20550934

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116589 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Transcription termination factor 1, mitochondrial Chain: O
Molecule details ›
Chain: O
Length: 343 amino acids
Theoretical weight: 39.32 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q99551 (Residues: 57-396; Coverage: 85%)
Gene names: MTERF, MTERF1
Sequence domains: mTERF
5'-D(*AP*TP*TP*AP*CP*CP*GP*GP*GP*CP*TP*CP*TP*GP*CP*CP*AP*TP*CP*TP*TP*A)-3') Chain: D
Molecule details ›
Chain: D
Length: 22 nucleotides
Theoretical weight: 6.68 KDa
Source organism: Homo sapiens
Expression system: Not provided
5'-D(*TP*AP*AP*GP*AP*TP*GP*GP*CP*AP*GP*AP*GP*CP*CP*CP*GP*GP*TP*AP*AP*T)-3') Chain: E
Molecule details ›
Chain: E
Length: 22 nucleotides
Theoretical weight: 6.83 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2221
Unit cell:
a: 88.631Å b: 89.709Å c: 161.293Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.205 0.244
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

19 review citations

Mitochondrial DNA mutations in disease and aging.
Park et al. (2011)
18 more

12 mentions without citation

Nucleic acid recognition by tandem helical repeats.
Rubinson et al. (2012)
11 more