› 3mwi

X-ray diffraction
2.03Å resolution

The complex crystal Structure of Urokianse and 5-nitro-1H-indole-2-amidine

Released:
DOI: 10.2210/pdb3mwi/pdb
PDB entry 3mwi coloured by chain, front view.
PDB entry 3mwi coloured by chain, side view.
PDB entry 3mwi coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Jiang LG, Yu HY, Yuan C, Huang ZX, Huang MD

Function and Biology Details

Reaction catalysed: 
Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133249 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Urokinase-type plasminogen activator chain B Chain: U
Molecule details ›
Chain: U
Length: 246 amino acids
Theoretical weight: 27.7 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P00749 (Residues: 179-424; Coverage: 60%)
Gene name: PLAU
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

2 bound ligands:
Ligand B25 1 x B25
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: R3
Unit cell:
a: 121.465Å b: 121.465Å c: 43.097Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.219 0.215 0.297
Expression system: Komagataella pastoris

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