› 3mxx

X-ray diffraction
2.05Å resolution

Crystal structure of WDR5 mutant (S62A)

Released:
DOI: 10.2210/pdb3mxx/pdb
PDB entry 3mxx coloured by chain, front view.
PDB entry 3mxx coloured by chain, side view.
PDB entry 3mxx coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The effect of Asp-His-Ser/Thr-Trp tetrad on the thermostability of WD40-repeat proteins.
Wu XH, Chen RC, Gao Y, Wu YD
Biochemistry 49 10237-45 (2010)
PMID: 20939513

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158474 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
WD repeat-containing protein 5 Chain: A
Molecule details ›
Chain: A
Length: 315 amino acids
Theoretical weight: 34.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61964 (Residues: 31-334; Coverage: 91%)
Gene names: BIG3, WDR5
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-002+
Spacegroup: P21
Unit cell:
a: 43.629Å b: 79.645Å c: 45.656Å
α: 90° β: 102.02° γ: 90°
R-values:
R R work R free
0.19 0.188 0.239
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Structure and function of WD40 domain proteins.
Xu et al. (2011)
1 more

2 mentions without citation

The architecture of the spliceosomal U4/U6.U5 tri-snRNP.
Nguyen et al. (2015)
1 more