› 3n3z

X-ray diffraction
2.75Å resolution

Crystal structure of PDE9A (E406A) mutant in complex with IBMX

Released:
DOI: 10.2210/pdb3n3z/pdb
PDB entry 3n3z coloured by chain, front view.
PDB entry 3n3z coloured by chain, side view.
PDB entry 3n3z coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Hou J, Luo H-B, Chen Y, Xu J, Zhao R, Zou L

Function and Biology Details

Reaction catalysed: 
Guanosine 3',5'-cyclic phosphate + H(2)O = guanosine 5'-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131212 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
High affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A Chains: A, B
Molecule details ›
Chains: A, B
Length: 326 amino acids
Theoretical weight: 38.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O76083 (Residues: 241-566; Coverage: 55%)
Gene name: PDE9A
Sequence domains: 3'5'-cyclic nucleotide phosphodiesterase
Structure domains: 3'5'-cyclic nucleotide phosphodiesterase, catalytic domain

Ligands and Environments

4 bound ligands:
Ligand IBM 2 x IBM
Ligand ZN 2 x ZN
Ligand MG 2 x MG
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P41212
Unit cell:
a: 104.09Å b: 104.09Å c: 270.21Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.201 0.23
Expression system: Escherichia coli BL21

Quick links