› 3n5c

X-ray diffraction
1.82Å resolution

Crystal Structure of Arf6DELTA13 complexed with GDP

Released:
DOI: 10.2210/pdb3n5c/pdb
PDB entry 3n5c coloured by chain, front view.
PDB entry 3n5c coloured by chain, side view.
PDB entry 3n5c coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
SAXS and X-ray crystallography suggest an unfolding model for the GDP/GTP conformational switch of the small GTPase Arf6.
Biou V, Aizel K, Roblin P, Thureau A, Jacquet E, Hansson S, Guibert B, Guittet E, van Heijenoort C, Zeghouf M, Perez J, Cherfils J
J Mol Biol 402 696-707 (2010)
PMID: 20709080

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158604 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ADP-ribosylation factor 6 Chains: A, B
Molecule details ›
Chains: A, B
Length: 162 amino acids
Theoretical weight: 18.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P62330 (Residues: 14-175; Coverage: 93%)
Gene name: ARF6
Sequence domains: ADP-ribosylation factor family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand GDP 2 x GDP
Ligand CL 3 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P212121
Unit cell:
a: 38.47Å b: 49.72Å c: 143.07Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.17 0.195
Expression system: Escherichia coli BL21

Quick links

Citations

3 review citations

Regulation of small GTPases by GEFs, GAPs, and GDIs.
Cherfils et al. (2013)
2 more