› 3n5u

X-ray diffraction
3.2Å resolution

Crystal structure of an Rb C-terminal peptide bound to the catalytic subunit of PP1

Released:
DOI: 10.2210/pdb3n5u/pdb
PDB entry 3n5u coloured by chain, front view.
PDB entry 3n5u coloured by chain, side view.
PDB entry 3n5u coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
An overlapping kinase and phosphatase docking site regulates activity of the retinoblastoma protein.
Hirschi A, Cecchini M, Steinhardt RC, Schamber MR, Dick FA, Rubin SM
Nat Struct Mol Biol 17 1051-7 (2010)
PMID: 20694007

Function and Biology Details

Reaction catalysed: 
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero trimer
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139029 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Serine/threonine-protein phosphatase PP1-alpha catalytic subunit Chains: A, B
Molecule details ›
Chains: A, B
Length: 300 amino acids
Theoretical weight: 34.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P62136 (Residues: 1-300; Coverage: 91%)
Gene names: PPP1A, PPP1CA
Sequence domains:
Structure domains: Purple Acid Phosphatase; chain A, domain 2
Retinoblastoma-associated protein Chain: C
Molecule details ›
Chain: C
Length: 13 amino acids
Theoretical weight: 1.53 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P06400 (Residues: 870-882; Coverage: 1%)
Gene name: RB1

Ligands and Environments

2 bound ligands:
Ligand MN 4 x MN
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P41212
Unit cell:
a: 92.946Å b: 92.946Å c: 192.381Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.221 0.261
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

30 review citations

Molecular mechanisms underlying RB protein function.
Dick et al. (2013)
29 more

8 mentions without citation

Signalling scaffolds and local organization of cellular behaviour.
Langeberg et al. (2015)
7 more