› 3n6s

X-ray diffraction
3.1Å resolution

Crystal structure of human mitochondrial mTERF in complex with a 15-mer DNA encompassing the tRNALeu(UUR) binding sequence

Released:
DOI: 10.2210/pdb3n6s/pdb
PDB entry 3n6s coloured by chain, front view.
PDB entry 3n6s coloured by chain, side view.
PDB entry 3n6s coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Human mitochondrial mTERF wraps around DNA through a left-handed superhelical tandem repeat.
Jiménez-Menéndez N, Fernández-Millán P, Rubio-Cosials A, Arnan C, Montoya J, Jacobs HT, Bernadó P, Coll M, Usón I, Solà M
Nat Struct Mol Biol 17 891-3 (2010)
PMID: 20543826

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero pentamer
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116603 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Transcription termination factor 1, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 353 amino acids
Theoretical weight: 40.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q99551 (Residues: 56-399; Coverage: 86%)
Gene names: MTERF, MTERF1
Sequence domains: mTERF
DNA (5'-D(*AP*TP*GP*GP*CP*AP*GP*AP*GP*CP*CP*CP*GP*GP*T)-3') Chain: B
Molecule details ›
Chain: B
Length: 15 nucleotides
Theoretical weight: 4.64 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*TP*AP*CP*CP*GP*GP*GP*CP*TP*CP*TP*GP*CP*CP*A)-3') Chain: C
Molecule details ›
Chain: C
Length: 15 nucleotides
Theoretical weight: 4.55 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P3121
Unit cell:
a: 83.244Å b: 83.244Å c: 171.858Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.239 0.237 0.287
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

8 review citations

Maintenance and Expression of Mammalian Mitochondrial DNA.
Gustafsson et al. (2016)
7 more

2 mentions without citation

Structural basis of mitochondrial transcription.
Hillen et al. (2018)
1 more