› 3n6w

X-ray diffraction
2Å resolution

Crystal structure of human gamma-butyrobetaine hydroxylase

Released:
DOI: 10.2210/pdb3n6w/pdb
PDB entry 3n6w coloured by chain, front view.
PDB entry 3n6w coloured by chain, side view.
PDB entry 3n6w coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human gamma-butyrobetaine hydroxylase.
Tars K, Rumnieks J, Zeltins A, Kazaks A, Kotelovica S, Leonciks A, Sharipo J, Viksna A, Kuka J, Liepinsh E, Dambrova M
Biochem Biophys Res Commun 398 634-9 (2010)
PMID: 20599753

Function and Biology Details

Reaction catalysed: 
4-trimethylammoniobutanoate + 2-oxoglutarate + O(2) = 3-hydroxy-4-trimethylammoniobutanoate + succinate + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131180 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Gamma-butyrobetaine dioxygenase Chain: A
Molecule details ›
Chain: A
Length: 403 amino acids
Theoretical weight: 46.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O75936 (Residues: 1-387; Coverage: 100%)
Gene names: BBH, BBOX, BBOX1
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand SO4 5 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P6522
Unit cell:
a: 164.8Å b: 164.8Å c: 97.78Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.21 0.208 0.233
Expression system: Escherichia coli

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Citations

5 review citations

Inhibition of 2-oxoglutarate dependent oxygenases.
Rose et al. (2011)
4 more

2 mentions without citation

Structure of the ribosomal oxygenase OGFOD1 provides insights into the regio- and stereoselectivity of prolyl hydroxylases.
Horita et al. (2015)
1 more