› 3n7q

X-ray diffraction
2.4Å resolution

Crystal structure of human mitochondrial mTERF fragment (aa 99-399) in complex with a 12-mer DNA encompassing the tRNALeu(UUR) binding sequence

Released:
DOI: 10.2210/pdb3n7q/pdb
PDB entry 3n7q coloured by chain, front view.
PDB entry 3n7q coloured by chain, side view.
PDB entry 3n7q coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Human mitochondrial mTERF wraps around DNA through a left-handed superhelical tandem repeat.
Jiménez-Menéndez N, Fernández-Millán P, Rubio-Cosials A, Arnan C, Montoya J, Jacobs HT, Bernadó P, Coll M, Usón I, Solà M
Nat Struct Mol Biol 17 891-3 (2010)
PMID: 20543826

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116606 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Transcription termination factor 1, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 310 amino acids
Theoretical weight: 35.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q99551 (Residues: 99-399; Coverage: 75%)
Gene names: MTERF, MTERF1
Sequence domains: mTERF
DNA (5'-D(*GP*GP*CP*AP*GP*AP*GP*CP*CP*CP*GP*G)-3') Chain: B
Molecule details ›
Chain: B
Length: 12 nucleotides
Theoretical weight: 3.71 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*CP*CP*GP*GP*GP*CP*TP*CP*TP*GP*CP*C)-3') Chain: C
Molecule details ›
Chain: C
Length: 12 nucleotides
Theoretical weight: 3.62 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CIT 1 x CIT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P32
Unit cell:
a: 99.851Å b: 99.851Å c: 39.6Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.231 0.228 0.278
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

7 review citations

Maintenance and Expression of Mammalian Mitochondrial DNA.
Gustafsson et al. (2016)
6 more

3 mentions without citation

Nucleic acid recognition by tandem helical repeats.
Rubinson et al. (2012)
2 more