› 3n9u

X-ray diffraction
1.92Å resolution

Crystal Structure of the Complex between the 25 kDa Subunit and the 59 kDa Subunit (RRM domain) of Human Cleavage Factor Im

Released:
DOI: 10.2210/pdb3n9u/pdb
PDB entry 3n9u coloured by chain, front view.
PDB entry 3n9u coloured by chain, side view.
PDB entry 3n9u coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Tresaugues L, Welin M, Arrowsmith CH, Berglund H, Bountra C, Collins R, Edwards AM, Flodin S, Flores A, Graslund S, Hammarstrom M, Johansson I, Karlberg T, Kol S, Kotenyova T, Moche M, Nielsen TN, Nyman T, Persson C, Schuler H, Schutz P, Siponen MI, Thorsell AG, van der Berg S, Wahlberg E, Weigelt J, Wisniewska M, Nordlund P, Structural Genomics Consortium (SGC)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129138 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cleavage and polyadenylation specificity factor subunit 5 Chains: A, B
Molecule details ›
Chains: A, B
Length: 230 amino acids
Theoretical weight: 26.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O43809 (Residues: 21-227; Coverage: 91%)
Gene names: CFIM25, CPSF25, CPSF5, NUDT21
Sequence domains: Nucleotide hydrolase
Structure domains: Nucleoside Triphosphate Pyrophosphohydrolase
Cleavage and polyadenylation specificity factor subunit 7 Chains: C, I
Molecule details ›
Chains: C, I
Length: 156 amino acids
Theoretical weight: 17.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8N684 (Residues: 50-182; Coverage: 28%)
Gene name: CPSF7
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits

Ligands and Environments

1 bound ligand:
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21212
Unit cell:
a: 165.95Å b: 124.73Å c: 40.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.196 0.231
Expression system: Escherichia coli BL21(DE3)

Quick links