› 3n9v

X-ray diffraction
2.65Å resolution

Function and Biology Details

Reaction catalysed: 
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H(2)O = 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152190 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type II inositol 1,4,5-trisphosphate 5-phosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 313 amino acids
Theoretical weight: 36.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P32019 (Residues: 341-646; Coverage: 31%)
Gene names: INPP5B, OCRL2
Structure domains: Endonuclease/exonuclease/phosphatase

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand CA 2 x CA
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: I212121
Unit cell:
a: 96.16Å b: 110.66Å c: 159.38Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.19 0.231
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

The extended human PTPome: a growing tyrosine phosphatase family.
Alonso et al. (2016)
4 more

3 mentions without citation

Inositol 5-phosphatases: insights from the Lowe syndrome protein OCRL.
Pirruccello et al. (2012)
2 more