› 3nbq

X-ray diffraction
2.3Å resolution

Human uridine phosphorylase 1 (hUPP1) with 5-fluorouracil

Released:
DOI: 10.2210/pdb3nbq/pdb
PDB entry 3nbq coloured by chain, front view.
PDB entry 3nbq coloured by chain, side view.
PDB entry 3nbq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Active site conformational dynamics in human uridine phosphorylase 1.
Roosild TP, Castronovo S
PLoS One 5 e12741 (2010)
PMID: 20856879

Function and Biology Details

Reaction catalysed: 
Uridine + phosphate = uracil + alpha-D-ribose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172590 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uridine phosphorylase 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 328 amino acids
Theoretical weight: 36.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q16831 (Residues: 1-310; Coverage: 100%)
Gene names: UP, UPP1
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

1 bound ligand:
Ligand URF 4 x URF
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P212121
Unit cell:
a: 61.317Å b: 85.281Å c: 260.581Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.222 0.277
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 citation in other articles

Primary cilia elongation in response to interleukin-1 mediates the inflammatory response.
Wann et al. (2012)
6 more

2 mentions without citation

Pathway analysis for drug repositioning based on public database mining.
Pan et al. (2014)
1 more